FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24681735
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG346950.1405695345161108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC237500.019.30358913
GGGATTA253200.019.14239112
GATTACA258900.018.16767514
ATTACAG281250.017.0103215
TACAGGC246200.016.46189917
TTACAGG288900.015.91791316
TGGGATT318050.015.82148411
ACAGGCG170150.015.16071218
AAAGTGC262350.014.5887134
GGCGTGA156200.013.93802821
CCAAAGT289750.013.7868292
ACCGCGC68000.013.74304431
CGTGAGC162450.013.72520323
AGGCGTG167200.013.69094820
CAGGCGT166800.013.68158719
CTACTCG40500.013.5602092
GCGTGAG160550.013.51678522
CGAGTAG43800.013.497323
CTGGGAT383100.013.38144510
AGTAGCT140400.013.2328125
Produced by FastQC (version 0.11.5)