FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938872
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGT450.004309488531.11066267
GCTAGTA808.636571E-526.2665581
CGGATAA750.001833361323.333610
ATCTAGT901.9297258E-423.33299669
ATATTAT901.9297258E-423.33299617
TACGCGT952.782632E-422.10494457
ACGTCGA3650.022.0687071
TTATCGA800.002668024321.8746852
TAGCTAA800.002668024321.87468568
GGATTAC14600.021.81475613
GATTACA15250.021.80296514
GGGATTA14900.021.61042812
ATTACAG15400.021.590615
AAAGTGC15400.020.6825894
ATGCGAA850.003790873320.5895
TGGGATT16050.020.49814811
ATTAACG900.00527858619.44416426
TTACAGG16850.019.3172316
ATACGCA1107.480379E-419.09063563
GCATAAT950.00721294518.42078640
Produced by FastQC (version 0.11.5)