FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938872
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35530.18325087989305122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAT450.004307302631.11388623
TAGTATT500.007208430728.00249954
TACCGAT1154.91018E-827.39021315
CGTATTA657.987204E-426.908122
ATTCGAC808.676035E-526.24895515
TATGCGA700.00122585225.00352137
CGATTTA1301.6078411E-724.23230718
TTTAGCG1703.759851E-922.6490821
ACCGATT1403.2906246E-722.49910516
GATTCTA1102.9239478E-522.27356317
GGCGTAT800.002666419821.87695128
TAACTAA800.002666819421.87638756
GATAATA800.00267041721.8713071
TGATACG800.002674419221.8656654
ATTTAGC1506.3845255E-721.00187320
TCAATTA850.0037897320.59007342
GCGTAAT850.0037897320.59007333
CGCGTAA850.003791996720.58794832
GTATTAG850.00380334820.5773283
TTAGCGT1751.2654345E-720.00178522
Produced by FastQC (version 0.11.5)