FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20381900
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC153900.026.72177313
GGGATTA173650.026.30278612
GATTACA168200.024.42907714
ATTACAG189550.021.43746415
TGGGATT232800.021.18352911
TTACAGG192400.020.48322316
ACGTCGA16100.020.4426611
TACAGGC168900.020.0176217
AAAGTGC173800.019.1112884
CTGGGAT292700.016.932110
ACAGGCG113950.016.89325518
CCAAAGT203750.016.8173072
AAGTGCT202000.016.5127455
CAAAGTG214550.016.1502843
GCGTGAG99700.015.69192622
CGTGAGC103800.015.17326523
GGCGTGA104700.015.00944421
CCCAAAG269550.014.977021
CGAGTAG32700.014.7708273
AGGCGTG112800.014.39706220
Produced by FastQC (version 0.11.5)