FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24346457
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC166300.026.07421513
GGGATTA188700.025.07353812
GATTACA180050.024.08308814
ATTACAG202600.020.93633815
TGGGATT258350.020.45291511
TTACAGG220200.019.13584516
TACAGGC188900.018.65759317
AAAGTGC191700.017.994244
CCAAAGT223500.017.247662
ACAGGCG127500.015.86708618
CTGGGAT336000.015.8196610
CAAAGTG234450.015.7709543
AAGTGCT228750.015.4791545
GGCGTGA116500.015.11195821
GCGTGAG119950.014.61897822
CGTGAGC122950.014.46190423
CCCAAAG310300.014.4439881
CAGGCGT126700.014.42027119
AGGCGTG130350.014.28496720
CGAGTAG38750.013.3605163
Produced by FastQC (version 0.11.5)