FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18901967
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC149300.026.95930713
GGGATTA167250.026.30509412
GATTACA159750.025.48052614
ACGTCGA17150.024.092041
ATTACAG178750.022.61534315
TGGGATT225150.021.48347311
TTACAGG189700.020.7749816
TACAGGC164450.019.89973417
AAAGTGC164950.018.6940064
CCAAAGT188050.017.51432
CTGGGAT278350.017.4654810
CGTCGAG25600.016.816732
CAAAGTG195950.016.6831953
ACAGGCG108650.016.52559918
AAGTGCT188350.016.2972325
CGAGTAG35000.015.5003413
GCGTGAG93300.015.26788522
CGTGAGC95250.015.10221623
ACCGCGC43300.015.03449631
CCCAAAG254800.014.7178971
Produced by FastQC (version 0.11.5)