FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18901967
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC157850.027.00514813
GGGATTA179700.025.823312
GATTACA171700.024.88815314
ATTACAG184750.022.69475215
TGGGATT229950.021.56327611
TTACAGG192300.021.29411716
TACAGGC168800.020.79668817
AAAGTGC175150.019.15494
CCAAAGT198050.018.4569052
CAAAGTG202800.017.6110633
ACAGGCG109600.017.56465718
CTGGGAT284400.017.44670710
AAGTGCT198250.017.1013455
GCGTGAG99750.016.77259422
CGTGAGC103050.016.40574323
GGCGTGA101950.016.03291921
CCCAAAG263150.015.5823741
AGGCGTG107900.015.37583420
CAGGCGT103150.015.2358219
CGGCTAA24300.015.12593839
Produced by FastQC (version 0.11.5)