FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24821867
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC274600.022.54548513
GGGATTA296850.022.2223512
GATTACA292300.021.22832514
TGGGATT357400.019.4352911
ATTACAG320100.019.25368515
TACAGGC280400.018.51035117
TTACAGG329350.018.160416
ACAGGCG174250.017.6157518
CTGGGAT411100.016.81956910
GGCGTGA154450.016.63382521
AAAGTGC283300.016.5475884
ACCGCGC80250.016.530131
GCGTGAG159850.016.13759422
AGGCGTG167700.016.05008120
CGTGAGC164050.015.9810523
CAGGCGT164250.015.896728519
CCAAAGT320500.015.5197612
AAGTGCT314850.015.0689295
CGGCTAA37100.014.90804139
CAAAGTG327800.014.8011953
Produced by FastQC (version 0.11.5)