FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26867139
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC267100.021.92105113
GGGATTA279600.021.89159212
GATTACA274600.021.18217514
ATTACAG296300.019.74912815
TGGGATT339250.019.12411911
TTACAGG314450.018.1305616
TACAGGC278650.017.6848217
AAAGTGC288800.016.741774
ACCGCGC92000.016.62541631
ACAGGCG197950.016.60306218
GGCGTGA183600.016.24253821
CGTGAGC188000.015.99313623
CCAAAGT320250.015.9369692
GCGTGAG188050.015.76517222
AAGTGCT317000.015.651475
AGGCGTG197500.015.648725520
CTGGGAT426750.015.4568910
CAAAGTG328400.015.0198093
CAGGCGT199100.014.96013819
CCCAAAG416000.013.7859261
Produced by FastQC (version 0.11.5)