FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29229068
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC215450.024.53022413
GGGATTA234900.024.37654312
ACGTCGA27650.022.9205721
GATTACA233200.022.8732714
ATTACAG259500.020.4741315
TGGGATT313000.019.63588511
TACAGGC242150.018.57320217
TTACAGG283100.018.30978816
AAAGTGC248650.016.7930854
CGGCTAA34700.016.54159239
CCAAAGT287800.015.7732712
ACAGGCG168750.015.74219718
CTGGGAT396800.015.63012210
CGTCGAG44200.014.7344061
CAAAGTG304200.014.7159783
GCGTGAG147850.014.65329322
ACCGCGC65400.014.4494231
AAGTGCT291350.014.3799585
CGAGTAG50950.014.1514453
GGCGTGA151600.014.12924221
Produced by FastQC (version 0.11.5)