FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29009995
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA272000.024.47434812
GGATTAC257200.024.0182313
GATTACA264200.023.5805814
ATTACAG282200.021.7415615
ACGTCGA29200.021.705681
TGGGATT341800.020.44923411
TTACAGG305700.019.93280216
TACAGGC289450.018.95993217
AAAGTGC275450.017.7133834
ACAGGCG212000.016.90551418
CCAAAGT305950.016.5308192
ACCGCGC96350.016.31018431
TGATCAA131950.016.233739
CTGGGAT445650.016.20231410
AAGTGCT313400.015.8924335
GCGTGAG195000.015.84849922
CAAAGTG322100.015.6043063
CGGCTAA42350.015.53697439
CGTGAGC200150.015.52813923
GGCGTGA193250.015.52113121
Produced by FastQC (version 0.11.5)