FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29009995
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG313900.10820408621235542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC242850.021.73177713
GGGATTA262750.021.48349212
GATTACA248100.021.21560114
ATTACAG270850.019.21440115
TGGGATT326300.018.4348611
TTACAGG291450.017.65219716
TACAGGC270850.016.20434217
AAAGTGC263100.015.4109924
CTGGGAT427950.014.48086810
CCAAAGT299550.014.2346312
ACAGGCG201900.013.95538318
AAGTGCT301500.013.8212895
CAAAGTG308350.013.7039353
GGCGTGA174300.013.07289521
GCTAATT118800.012.9873151
CGGCTAA37850.012.85527239
ACCGCGC87650.012.77851131
CCCAAAG402350.012.4953941
AGTAGCT160000.012.3990175
GCGTGAG186550.012.34585222
Produced by FastQC (version 0.11.5)