FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27816697
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG314860.1131910089828422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA282500.021.26957712
GGATTAC268150.020.85558513
GATTACA290900.019.62170614
ATTACAG318100.017.98800315
TGGGATT358900.017.93996811
TACAGGC280300.017.38090317
TTACAGG330950.016.6022816
ACAGGCG188950.015.57866818
AAAGTGC297150.015.2462974
CGAGTAG48050.015.0697453
CTGGGAT425650.014.92084910
AGTAGCT164150.014.7926475
GGCGTGA167400.014.57360921
CTACTCG44100.014.5154242
CCAAAGT337750.014.36475852
GCGTGAG174350.014.09307422
CGGCTAA39600.013.96707939
AAGTGCT337100.013.8252445
AGGCGTG187500.013.81402620
CAGGCGT180700.013.81060119
Produced by FastQC (version 0.11.5)