FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12419505
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG287510.23149875941110373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC94550.025.68819613
GGGATTA107950.025.12469112
GATTACA100550.024.05091714
ATTACAG110050.020.92545915
TGGGATT143450.020.83287811
TTACAGG115900.019.26540616
TACAGGC106150.018.06873317
AAAGTGC101850.017.8971084
CCAAAGT119300.016.7732142
CTGGGAT179150.016.46628610
AAGTGCT117250.015.8164665
CAAAGTG124700.015.4576743
ACAGGCG70550.015.18136518
CGGCTAA15500.015.13113539
GCGTGAG61000.015.033360522
ATCGCTT17900.014.27423127
CCCAAAG164750.014.2515141
AATCGCT14750.014.23783226
CGTGAGC61650.014.13696423
GGCGTGA62750.014.11209421
Produced by FastQC (version 0.11.5)