FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24764584
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA168400.022.06941212
GGATTAC155750.022.06540513
GATTACA160550.021.29693614
ATTACAG176500.019.11502315
TGGGATT235150.018.08028811
TTACAGG198850.016.57936116
TACAGGC186700.015.20313417
AAAGTGC190100.015.0346674
CCAAAGT213800.013.8733552
CTGGGAT335750.013.02734110
AAGTGCT219750.012.9440965
CAAAGTG229350.012.7956083
ACAGGCG146200.011.994060518
GGCGTGA124800.011.97556921
ATCGCTT30750.011.95174927
GCTAATT79400.011.68291641
CGTGAGC131950.011.67176923
GCGTGAG129850.011.64467322
CCTATAT30150.011.2559961
CCCAAAG316300.011.1828231
Produced by FastQC (version 0.11.5)