FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26926468
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC203650.021.29193313
GGGATTA219900.020.83201812
GATTACA210350.020.66377814
ATTACAG222300.019.25410315
TGGGATT293100.017.2399211
TTACAGG247100.016.93930216
TACAGGC233300.015.7367417
AAAGTGC229300.014.87601954
CCAAAGT255600.014.205952
ATCGCTT38750.013.81985927
ACAGGCG163950.013.62009918
CTGGGAT392000.013.55961810
AAGTGCT265800.013.3217395
CAAAGTG274300.013.03362753
ACCGCGC75800.012.79070431
GGCGTGA148000.012.581470521
GCTAATT97150.012.4612921
AATCGCT36050.012.2335726
AGTAGCT137600.012.1801475
GGCTAAT67750.012.03825940
Produced by FastQC (version 0.11.5)