FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26084805
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA29250.024.4211981
GGGATTA269450.023.45888912
GGATTAC252200.023.3564113
GATTACA261050.022.76570114
ATTACAG278050.021.36125815
TGGGATT323300.020.59093311
TTACAGG302100.019.35943816
CGTCGAG51000.018.6750351
AAAGTGC259300.018.5870724
TACAGGC280050.018.07166117
TGATCAA139550.017.6569169
CCAAAGT289450.017.0378682
GTCGAGA57600.016.6496832
AAGTGCT297850.016.4987685
CAAAGTG300500.016.353183
CTGGGAT416900.016.12748510
ACAGGCG206200.015.98923318
TTGTGAT158200.015.7746326
GGCGTGA187700.015.756421
CGTGAGC195450.015.52552523
Produced by FastQC (version 0.11.5)