FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29034602
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA229500.022.58603312
GGATTAC218950.022.25163313
GATTACA237450.020.7979714
ACGTCGA28850.020.2691611
ATTACAG261450.018.59429615
TGGGATT306400.018.18542311
TACAGGC242950.017.60433817
TTACAGG276450.017.23088616
CGTCGAG45550.015.2209541
AGTAGCT163000.015.095575
AAAGTGC249800.014.9924424
CGGCTAA36050.014.56291739
CTGGGAT394050.014.54910
CGAGTAG51500.014.4081493
ACAGGCG175150.014.3276318
CCAAAGT287550.014.1561012
TGATCAA153100.014.082439
GCTAATT135950.013.41284541
GACTTGT164400.013.3914743
TAGCTGG203250.013.2424357
Produced by FastQC (version 0.11.5)