FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005399748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005399748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29034602
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTA253250.024.1692512
GGATTAC237850.023.96927813
GATTACA257850.022.58516314
ATTACAG280750.020.60590215
TGGGATT330600.019.76229311
TTACAGG293900.018.99323316
TACAGGC258500.018.33220317
AAAGTGC266400.016.4947814
CCAAAGT305100.015.8107542
CTGGGAT422500.015.66221610
ACAGGCG178600.015.40336218
AGTAGCT161600.014.9832995
AAGTGCT307900.014.5914385
CAAAGTG316250.014.5679763
CGGCTAA38000.014.554728539
AATCGCT39850.014.31693926
ACCGCGC70500.014.00032631
GGCGTGA158450.013.80610621
GGCTAAT90550.013.80066140
GCGTGAG163650.013.7952222
Produced by FastQC (version 0.11.5)