FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457206
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT23911216.408936004929984No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG21102914.481754810232733No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA17415411.951227211526716No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC1222748.390989331638767No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG897036.155821483029853Illumina Paired End PCR Primer 2 (100% over 31bp)
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC460283.1586474390031336No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT429462.94714680010925No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA320752.2011301078914034No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT256391.7594629722908084No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA251941.7289250799132037No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT245341.6836329249261945No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA210971.4477705966074803No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT159961.0977171381397002No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA152231.044670417223097No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC132410.9086567033075625No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA121590.8344050189197684No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG103050.7071752380926238No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC101530.6967443175501611No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT84230.5780239719023941No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA78880.5413098765720152No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT77450.5314965763248298No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGNCACTGTCT67360.4622544787765079No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTACA62920.4317852108761562No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT62730.4304813458083483No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCANCTGGACAG62390.42814811358174476No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAGAGGCCTGAGGGATCCATCTC57560.3950024910685243No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA55770.3827187096402293No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATNCAATTTCA51540.3536905557621915No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTATA51060.35039658085404535No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT47220.32404478158887623No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACATGACTAC41170.2825269728507843No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC38220.26228275206113616No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGANCCAATTTC37130.2548026840405543No Hit
TGACTCTGAGATGCTCTCCTAAGTCTGGGCATGACACTGTGTCCTGGTAC34570.23723481786377493No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATACAATTTCA34210.23476433668266533No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGTGCACCAGACAAATCA32160.22069631884579116No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGCTATGAGAAACTCTCTAT30380.2084811618947493No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA26630.18274698292485755No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCANGAATGCCG24920.17101219731458697Illumina Paired End PCR Primer 2 (96% over 31bp)
CAAAGAGGGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTAGACAAAT21800.14960136041163707No Hit
CAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT21600.14822887086657618No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGATCCGTCTC19240.13203349423485766No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCGACTGGACAG19120.13121000050782114No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGAACCAATTTC16300.11185789792246258No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGCAATGCCG16280.11172064896795648Illumina Paired End PCR Primer 2 (96% over 31bp)
TGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGAGA16250.11151477553619736No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATAACTCCATG15490.10629931526496597No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACTACACTGTCT15270.10478957676539899No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA14690.10080935708472241No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCTGG241150.0292.241881
GCCACTC266750.0291.530828
TTGCGCT34200.0291.18759
GAGTCAC241850.0289.811467
GATCTTG34500.0288.58615
GGAGTCA243650.0288.114566
AACAGCC270300.0288.078644
TTCTGGA244350.0288.053042
TCAATGG50200.0287.88399
GTCACAC243900.0287.204229
ACAGCCA271200.0286.959725
ATCTTGC34750.0286.953466
ATCTACC110850.0286.856545
AGTCACA244450.0286.487958
TCTTGCG34800.0286.157177
TGATCTT34800.0286.098274
CTTGCGC34850.0285.746648
ATTCATC50750.0285.276034
TCTACCA111700.0284.825046
AAACAGC273500.0284.708073
Produced by FastQC (version 0.11.5)