FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005400919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590440
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT13097322.18227084885848No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA8742814.807262380597521No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG547739.276641148973647No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG537909.11015513854075Illumina Paired End PCR Primer 2 (100% over 31bp)
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC390466.613034347266446No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA327005.538242666486011No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT232663.9404511889438387No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC227483.8527200054196866No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA89021.5076891809498No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT69731.1809836731928731No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT37480.6347808414064088No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGNCACTGTCT37390.6332565544339814No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA33350.5648330058939097No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC33320.5643249102364338No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG32200.5453560056906713No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATNCAATTTCA25770.43645416977169565No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT25640.43425242192263397No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT19090.32331820337375516No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATACAATTTCA16840.2852110290630716No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA16800.2845335681864372No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCANCTGGACAG16220.2747103854752388No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCANGAATGCCG14400.24388591558837475Illumina Paired End PCR Primer 2 (96% over 31bp)
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT13620.2306754284940045No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC13510.22881241108325995No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGTGCACCAGACAAATCA13440.2276268545491498No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATAACTCCATG12850.2176343066187928No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA12240.20730302825011857No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGANCCAATTTC11740.19883476729218888No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA11600.19646365422396855No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA11150.18884221936183185No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA10770.1824063410338053No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCANGACACTGA10180.1724137931034483No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGCAATGCCG10030.16987331481606938Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGG8930.15124314070862407No Hit
AGGAACACCAGTGACTCTGAGATGTCATCAGACTGAGAACCACCGCTATA8830.14954948851703814No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA8780.14870266242124516No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT8270.1400650362441569No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACTACACTGTCT7730.13091931440959284No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAAGAATGCCG7230.12245105345166317Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACNTCTATTGT7010.11872501863017411No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGTCACTGTCT6970.11804755775353974No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAANAAGTTAAC6890.116692636000271No Hit
AACAAGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAAT6700.1134746968362577No Hit
CTGATGTTACCCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCC6580.11144231420635459Illumina Paired End PCR Primer 2 (100% over 30bp)
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTACA6120.10365151412505928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)