FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005400920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590440
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT526098.91013481471445Illumina Single End PCR Primer 1 (100% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGCAGAGCGTCGTGTAGGGAAAGAGT14110.23897432423277556Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTCGGGAAAGAGT7290.12346724476661472Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGTAGATGTCAGGATCGGAAGAGCGTCGTGTAGGGAAAGAGT6750.11432152293205068Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGGTAACATCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG6270.10619199241243818Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)