FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166602
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC4656927.952245471242843No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG2150512.908008307223204No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT2043312.264558648755717No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA1719310.319804083984586No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG120967.2604170418122225Illumina Paired End PCR Primer 2 (100% over 31bp)
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA65623.938728226551902No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC46612.797685501974766No Hit
GATCACAGAGACAGGAAGGCAGACATAGAGCACCAGGCAGACCAACCAAA40312.4195387810470463No Hit
ACTATTCATCAATGGCCAGCGAGGTGCTCCGTGTGCTCCACAAGGAGCCG26711.6032220501554604No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG21811.3091079338783447No Hit
ATTCTGGAGTCACACAAACCCCAGTCCCTTCTCCCTTCCTCCTGAATGTC20341.22087369899521No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC15450.9273598156084562No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT14020.8415265122867672No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA13920.8355241833831527No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT13230.7941081139482119No Hit
GGAAACAGCCACTCTGAAATGCAGCCAACCCATGGTAAGCGAGCCAGAAC11320.6794636318891729No Hit
GATCACAGAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGA9920.5954310272385686No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT7910.47478421627591505No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC7480.44897420199037225No Hit
AAGAAGCCACTGGAGCTCATGTTGTCTACAACTTTAAAGAACAGACTGAA5830.3499357750807313No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC5440.3265266923566344No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT5410.32472599368555No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTACCCAGGATATGAACC4830.2899124860445853No Hit
GAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGCG4550.2731059651144644No Hit
TGACTCTGAGATGCTCTCCTAAGTCTGGGCATGACACTGTGTCCTGGTAC4190.2514975810614518No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT3040.18247079866988392No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA2780.16686474352048594No Hit
AGGAACACCAGTGACTCTGAGATGTCATCAGACTGAGAACCACCGCTATA2730.16386357906867866No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGG2630.15786125016506403No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA2570.15425985282289528No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA2190.1314510029891598No Hit
AAGTGATCTTGCGCTGTGTCCCTCCCATGTGGCGTCCCCGCCAGCTGTTG2100.12604890697590665No Hit
TGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGAGA2050.12304774252409935No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTACCCAGGATATGAACCAT1890.11344401627831598No Hit
CATGAGGTGACAGAGATGGGACAAGAAGTGACTCTGAGATGTAAACCAAT1790.10744168737470139No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGCG1150.0295.07
ATGAGGT305.456968E-12295.02
GATCTTG1150.0295.05
TCCCAGG108.458544E-4295.0295
TGACCCA50600.0292.959472
GCCACTC22450.0292.371928
ATTCTGG25750.0291.56311
CATCTAC13750.0290.709084
ACCCAGA51050.0290.377084
ATCTACC13800.0289.65585
GAATCTT2600.0289.32693295
GTGACCC51250.0289.24391
ACAGCCA22900.0288.558965
TCAATGG4500.0288.444469
AACAGCC22950.0287.93034
TACCAGA13900.0287.571938
AAACAGC23000.0287.304353
CCAGAAC51650.0285.004856
GTCGCAA5150.0283.54374
GAGTCAC26550.0283.33337
Produced by FastQC (version 0.11.5)