FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412633
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT17669442.82110252936629No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG405159.81865241025318Illumina Paired End PCR Primer 2 (100% over 31bp)
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG385849.350682083110174No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC229835.569840512028849No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC215255.2164998921559835No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA185444.494066155639516No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGNCACTGTCT49811.2071259448468736No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA37750.9148565432236393No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT35970.8717189366822333No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA23130.5605465389341133No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT23050.5586077701007918No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA20500.4968095135386651No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG19470.47184786480964924No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT16740.405687378372549No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT15330.3715165776852554No Hit
TGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGAGA13680.331529470497997No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCANCTGGACAG11580.2806367886233045No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCANGAATGCCG11230.27215467497752244Illumina Paired End PCR Primer 2 (96% over 31bp)
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACTACACTGTCT10970.2658536762692271No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC10120.24525425741518492No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA10000.24234610416520247No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA9760.23652979766523763No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC9730.23580275935274203No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGTCACTGTCT9310.2256242229778035No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA8120.1967850365821444No Hit
GATCACAGAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGA7430.18006315539474546No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACCACACTGTCT7380.17885142487391945No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGCAATGCCG7240.1754585794156066Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGA7080.17158104174896338No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGANCCAATTTC6620.16043312095736403No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAANAAGTTAAC6160.14928520016576474No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACAACACTGTCT5850.14177247093664344No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAAGAATGCCG5710.13837962547833063Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGG5550.13450208781168738No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA5530.134017395603357No Hit
CTGATGTTACCCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCC5520.13377504949919178Illumina Paired End PCR Primer 2 (100% over 30bp)
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT5470.13256331897836576No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATNCAATTTCA5440.13183628066587016No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGCTATGAGAAACTCTCTAT5440.13183628066587016No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT5190.1257776280617401No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGCCACTGTCT5120.12408120533258368No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTATA5030.12190009039509686No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT4940.11971897545761004No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACGCGACACTGTCT4550.11026747739516712No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT4280.10372413258270667No Hit
GATCACAGAGACAGGAAGGCAGATCGGAAGAGCGGTTCAGCAGGAATGCC4210.10202770985355024Illumina Paired End PCR Primer 2 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACTC201950.0293.129278
AACAGCC203150.0291.87184
ACAGCCA203800.0290.72415
AAACAGC204050.0290.512273
CAGCCAC203850.0290.436046
CTTGCGC6500.0290.128058
TTGCGCT6500.0290.128059
ATCTTGC6500.0290.022586
GAAACAG205700.0288.396732
ATCTACC46600.0287.91725
TCTACCA46600.0287.91726
GATCTTG6550.0287.808655
GAGTCAC45200.0285.825937
TCTTGCG6600.0285.697547
TGACATC47350.0284.60091
ATTCTGG45650.0284.55291
AGCCACT208100.0284.431927
TACCAGA47150.0284.34978
GTCACAC45550.0283.017159
CTACCAG47450.0282.828127
Produced by FastQC (version 0.11.5)