FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005400926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412633
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT391869.496574437817625Illumina Single End PCR Primer 1 (100% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGCAGAGCGTCGTGTAGGGAAAGAGT11880.2879071717482605Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTCGGGAAAGAGT5880.14249950924913907Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGGTAACATCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5470.13256331897836576Illumina Single End PCR Primer 1 (100% over 30bp)
TGGGGTCTGGTAGATGTCAGGATCGGAAGAGCGTCGTGTAGGGAAAGAGT5390.13062455014504415Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)