FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691884
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT656769.492342647033318Illumina Single End PCR Primer 1 (100% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGCAGAGCGTCGTGTAGGGAAAGAGT23220.33560539049898536Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTCGGGAAAGAGT7880.11389192407975904Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGA74800.0291.828348
GGTAGAT74950.0291.047529
TCTGGTA76500.0286.114446
CTGGTAG76850.0284.043737
TTGGTAA5550.0265.76987295
GGGTCTG86000.0256.223823
GGGGTCT86100.0255.754932
GTACCAA16550.0251.33319295
TGGGGTC91050.0249.41171
CTGGTAA9450.0243.49583295
GTTACGG450.0229.43144
GGTCTGG96850.0227.367054
CAGGCTA7900.0197.91443295
GGATGGT1000.0191.73918
TAGTGGG850.0190.87158
CGTGCTT700.0189.63214
TCGGTAA1900.0186.31866295
GGCCTGT13800.0184.91228295
GGCGTGA1300.0181.528157
GCGGTTT255.5568074E-5176.989947
Produced by FastQC (version 0.11.5)