FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245452
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT58618847.066285974891045No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1210909.722574615480966Illumina Paired End PCR Primer 2 (100% over 31bp)
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC1035508.314250569271236No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA754006.054026971733957No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG680165.461149847605528No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT339842.7286479125650764No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA301962.4245013055501135No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT281432.2596615525929544No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA195651.570915619389587No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC127501.0237247200213255No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT114380.9183814390277587No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT108730.8730163828072058No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA82380.6614466073361318No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT82030.6586363826145046No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA70620.567023056689459No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTACA61800.49620539370445427No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAGAGGCCTGAGGGATCCATCTC53830.4322125621862584No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC40200.3227743823126062No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGA37340.29981083173016704No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT27770.22297125862738987No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC24690.1982412810770708No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGCTCTCAGGTGTGATCCAATTTCG22040.17696386532760797No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG20050.16098573048178494No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGATCCGTCTC18120.1454893484453837No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGTGCACCAGACAAATCA17400.1397083147323221No Hit
CAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT17260.13858422484367122No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTAGACAAAT16510.13256231472589872No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGTGATCGCTTCTTTGC16260.13055501135330788No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA15700.1260586517987044No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGAGCTCAGGTGTGATCCAATTTC14740.11835060684795559No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGG13890.11152577538114676No Hit
TGACATCTACCAGACCCCAAGATCGGAAGCGCGGTTCAGCAGGAATGCCG12500.10036516862954173Illumina Paired End PCR Primer 2 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCGC41050.0293.551158
TTGCGCT41100.0293.1949
GCCACTC609300.0292.616068
AACAGCC611200.0291.97124
AGCCACT611100.0291.802437
ATCTTGC41350.0291.07646
TCTTGCG41400.0290.713177
ACAGCCA613900.0290.651375
AAACAGC615150.0290.336153
AAAGTCG179150.0290.236541
CAGCCAC615550.0289.84836
GTCGCAA179650.0289.83934
ATTCTGG70150.0289.334721
GATCTTG41650.0288.97985
AGTCGCA180050.0288.867653
TGATCTT41750.0288.65254
GAAACAG619250.0288.532962
TCGCAAA180150.0288.286355
CGCAAAG180350.0287.80316
TTCTGGA70300.0287.668242
Produced by FastQC (version 0.11.5)