FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268221
Sequences flagged as poor quality0
Sequence length301
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA7494327.94076526446475No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT3465812.92143419046234No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG236868.830777605034653No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC190027.084456474325276No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA85433.1850600810525647No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT78252.917370377412656No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC63722.3756529130828685No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA48581.8111930087502468No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT48061.7918060107150449No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA42591.5878697044601282No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA36931.3768496873846565No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT35571.3261452309848967No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT31661.180369918835587No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT30291.1292926355505348No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGTGCACCAGACAAATCA28771.0726229489860972No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA28731.071131641444928No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATNCAATTTCA22600.8425887607607159No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG22370.8340137423989918Illumina Paired End PCR Primer 2 (100% over 31bp)
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC15670.5842197292531159No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC15440.5756447108913918No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATACAATTTCA15270.5693066538414218No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA13400.499588026291752No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG12360.4608140302213473No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGNCACTGTCT9960.3713355777511828No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT9460.35269423348656515No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA7810.29117779741332706No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCANCTGGACAG7320.2729092800340018No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGATCCGTCTC6960.2594875121634771No Hit
AGGAACACCAGTGACTCTGAGATGTCATCAGACTGAGAACCACCGCTATA6930.2583690315076001No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTACA6230.2322711495371354No Hit
GATCACAGAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGA5990.22332330429011898No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGANCCAATTTC5810.21661242035485662No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT5340.1990895567461161No Hit
AACAAGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAAT5220.19461563412260785No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGTCTCCACT4930.18380365444912963No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTAGACAAAT4760.17746559739915965No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATGCAATTTCA4650.17336450166094378No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA4260.15882425313454204No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGCACCAATTTCA3730.13906442821404735No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT3470.12937092919644622No Hit
TGACTCTGAGATGCTCTCCTAAGTCTGGGCATGACACTGTGTCCTGGTAC3450.1286252754258615No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGAGAGGACTGGGGGATC3360.12526983345823034No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA3040.11333937312887506No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAGAGGCCTGAGGGATCCATCTC3030.11296654624358272No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCAAATTTCA2980.11110241181712095No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACATGACTAC2860.10662848919361272No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGTGATCGCTTCTTTGC2740.10215456657010451No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTCA255.347829E-10294.642369
ATCTACC2250.0294.587435
CACGGTT108.4969547E-4294.58743295
ATCTTGC4800.0294.587436
GCCACTC39500.0291.658638
TTGCGCT4900.0291.635779
GATCTTG4850.0291.550455
TGATCTT4850.0291.550454
ACAGCCA39550.0291.235635
ATTCTGG26550.0290.703981
TCAATGG3450.0290.37229
AGGTCAC102350.0289.838351
GTCACAC26600.0289.103979
AACCTCT5000.0288.695681
CTGTGAA4950.0288.636176
GAGTCAC26700.0288.572977
TCGCAAA42900.0288.063935
GTCGCAA43050.0287.74454
AGTCGCA43100.0287.410743
CAGAGAA102600.0287.175787
Produced by FastQC (version 0.11.5)