FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005400933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315999
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA22783917.3130070767531No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT22488917.08884277267688No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC19796915.043248513106771No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG17647613.410040585137223No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC1106758.409960797842551No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG1076258.178197703797647Illumina Paired End PCR Primer 2 (100% over 31bp)
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT194961.481460092294903No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA188631.4333597517931245No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT165521.2577517156168052No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT157721.1984811538610591No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA129960.9875387443303528No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC116230.8832073580603025No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA79930.6073712821970229No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG77250.5870065250809461No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT59110.4491644750489932No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA59080.4489365113499327No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGAGCATGACACTGA57400.43617054420254114No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT57120.43404288301130933No Hit
TGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGAGA48160.3659577248918882No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC46360.35227990294825456No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA40850.31041057022079804No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGCTCTCAGGTGTGATCCAATTTCG35170.26724944319866506No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA33030.2509880326656783No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGAGCTCAGGTGTGATCCAATTTC32300.24544091598853798No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA32270.24521295228947743No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT29920.22735579586306678No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT27360.20790289354323216No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT22920.17416426608226906No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTATA22190.16861714940512873No Hit
GATCACAGAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGA21980.1670214035117048No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC20920.15896668614489828No Hit
CAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT19780.15030406558059695No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATAACTCCATG19660.1493922107843547No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCG19080.14498491260251717No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGCTATGAGAAACTCTCTAT16670.1266718287779854No Hit
AGGAACACCAGTGACTCTGAGATGTCATCAGACTGAGAACCACCGCTATA14970.11375388583122023No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAGAGGCCTGAGGGATCCATCTC14110.1072189264581508No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA13680.10395144677161608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)