FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315999
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1010007.6747778683722405Illumina Single End PCR Primer 1 (100% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGCAGAGCGTCGTGTAGGGAAAGAGT38520.2927053895937611Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTCGGGAAAGAGT13180.10015205178727339Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGA119450.0289.171088
GGTAGAT121150.0284.991679
CTGGTAG123550.0279.694347
TCTGGTA125350.0277.442936
GGGGTCT139150.0250.563842
GGGTCTG139700.0248.838353
TGGGGTC146950.0247.17631
TTGGTAA5700.0214.77829295
CTGGTAA14650.0202.36974295
TCGGTAA4250.0201.29185295
CTGTTGG68250.0176.7819295
TTGGGTG9300.0173.61991
TCGCATG2950.0164.985616
TATGGGT1650.0160.895079
TTGCGGT3400.0156.162865
TGGCGTG12200.0154.74066
GTGAGGT6300.0154.510355
TTGCGTG3300.0151.956456
TCGCTTG3450.0149.624636
GGTGAGG4250.0149.222274
Produced by FastQC (version 0.11.5)