FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538504
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT11688221.704945552864974No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC11389321.149889323013387No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA6304111.706691129499502No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA367566.825576040289394No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG260494.837289973704931No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC189703.5227222081915825No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT178753.319381100233239No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT170923.1739782805698753No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA93121.7292350660347928No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA87951.633228351135739No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT71261.3232956486859893No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT62461.1598799637514299No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA56161.042889189309643No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTACA49150.9127137402879086No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC47990.8911725818192622No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT45720.8490187630918248No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT45000.8356483888699062No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC40360.7494837549953204No Hit
AGGAACACCAGTGACTCTGAGATGTCATCAGACTGAGAACCACCGCTATA33230.617079910269933No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA27070.5026889308157414No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG22950.42618067832365214No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGTGCACCAGACAAATCA21100.39182624455900045No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACATGACTAC20720.384769658164099No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT19710.36601399432501897No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG17790.3303596630665696Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA15170.28170635687014395No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT14970.27799236403072214No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGTGATCGCTTCTTTGC13860.25737970377193115No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA12070.22413946785910596No Hit
CAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT11190.20779789936565002No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTACTCACTTATACTTCTATTGT11090.20594090294593914No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGAGAGGACTGGGGGATC10730.1992557158349799No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA9100.16898667419369215No Hit
TGACTCTGAGATGCTCTCCTAAGTCTGGGCATGACACTGTGTCCTGGTAC7970.14800261465095896No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA7730.14354582324365278No Hit
GATCACAGAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGA7550.14020322968817314No Hit
CATGAGGTGACAGAGATGGGACAAGAAGTGACTCTGAGATGTAAACCAAT7540.14001753004620207No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTAGACAAAT7050.13091824758961865No Hit
GACAGAGCATGACACTGCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG6370.11829067193558451No Hit
GACAGAGCATGACACTGCTGTGTGTGTGTGTGTGTGGGGAGGTAGGGCTG6150.11420527981222052No Hit
GGAAACAGCCACTCTGAAATGCTATCCTACCCCTAGACACGACACTGTCT5890.10937708912097217No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTT157.238228E-6295.0295
ACAGCCA118650.0293.25965
GCCACTC118500.0293.25748
AACAGCC118700.0293.136054
ATCTTGC19000.0292.671086
GATCTTG18950.0292.66495
AACCTCT38800.0292.338931
AAACAGC119400.0292.282233
TTCTGGA25800.0291.569762
AAAGTCG144600.0291.225771
TGATCTT19050.0291.12864
GTCGCAA144500.0291.019044
AGTCGCA144600.0290.919773
TCGCAAA144150.0290.70245
ATTCATC6600.0290.53034
TATTCAT6600.0290.53033
CATCAAT6550.0290.49627
TCTTGCG19150.0290.378577
TTGCGCT19050.0290.35439
CGCAAAG144300.0290.195776
Produced by FastQC (version 0.11.5)