FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538504
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT14370.26685038551245677Illumina Single End PCR Primer 1 (100% over 31bp)
TCGGCTGAGGTTGCGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA7160.13296094365130062No Hit
TTAGATGCAGTACTCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA7120.13221814508341628No Hit
TCAACTGTATTGACATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA7080.13147534651553192No Hit
TCCCTTTCCTTACGATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA6770.1257186576144281No Hit
TGCTTTCGGCTCCTTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA6430.11940486978741105No Hit
TGTAGTGAGGTGCGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA6110.11346248124433617No Hit
TTTTGTGTTTTGTAGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5890.10937708912097217No Hit
TTGGGTGTGGTGGGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5880.10919138947900109No Hit
TGCCATTAGATACACTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5670.1052916969976082No Hit
TGTGGTACGGTTTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5600.10399179950381056No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAG206.1818355E-8294.99993
GGGCTAT108.463787E-4294.99992
GGTCGAA108.463787E-4294.99994
TTTCGCA108.463787E-4294.99999
GTTACGG800.0294.99994
TCGTGCG600.0294.99998
AATCGGG157.238239E-6294.99994
GGACTAT700.0273.928472
TACGTGC600.0270.416567
GGTAACG550.0268.181734
TGGTAGA2600.0266.634528
GTTCGCA500.0265.49994
CAGGTCG450.0262.222147
TATGACG400.0258.124944
CGTGACA750.0255.666589
GTTAGCG351.2732926E-11252.857069
GATGGAA351.2732926E-11252.857064
TACGTAC351.2732926E-11252.857067
TGGTAGG650.0249.61538
GTATCAA950.0248.42099295
Produced by FastQC (version 0.11.5)