FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005400937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479196
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG26959756.260277631699765No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT7510215.672501439911851No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG288496.020292322974315Illumina Paired End PCR Primer 2 (100% over 31bp)
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC234994.9038389302080985No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC170053.5486523259793485No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA94091.9634971911284735No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT53351.1133231496089282No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG30870.6442040417699647No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC25940.5413233833337506No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT23400.48831793253699946No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC19140.39941902687000724No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA14550.30363358625698045No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA14310.2986251972053189No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT14010.292364710890742No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACATGACTAC13200.27546139784138435No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA11220.23414218816517665No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA11090.23142931076219334No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC10060.20993497441547923No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT8710.18176278599988313No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGCTCACAGCAACTGGACAG8540.17821517708828954No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT7980.16652893596774598No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTACA7450.1554687434786601No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA6140.12813128657167422No Hit
GATCACAGAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGA5570.11623636257397807No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)