FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479196
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT268105.5947879364602375Illumina Single End PCR Primer 1 (100% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGCAGAGCGTCGTGTAGGGAAAGAGT10430.2176562408701241Illumina Single End PCR Primer 1 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTG108.4649865E-4294.982579
GGTAGAT32350.0288.599649
TGGTAGA32450.0287.255778
CTGGTAG33250.0279.013587
GGGGTCT34300.0277.782442
TCTGGTA33750.0277.502146
GGGTCTG34800.0272.519843
TTGGTAA5900.0272.51233295
TGGGGTC38350.0269.57091
CTGTTGG159150.0252.28601295
GTACCAA3750.0243.87773295
TTCGTGC550.0241.349387
GCGTGAT800.0239.673348
GCGATTT251.8815808E-7235.986052
ATGTCGC700.0231.7728
CTATGAG450.0229.43098
CGCTTAA450.0229.43097
GTCGCGT2000.0228.611485
GATATCG650.0226.909674
GTGTGTC1500.0226.153323
Produced by FastQC (version 0.11.5)