FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005400939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533348
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC25988316.948729186068658No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG19543712.745769388292807No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC18775512.244774180420883No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG16167910.544181751304988Illumina Paired End PCR Primer 2 (100% over 31bp)
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA965606.297331069007166No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT835495.448795707171496No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA388162.53145404696129No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT371632.4236507302973624No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT266371.7371790356787893No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT184851.2055319470857235No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT169651.106402460498204No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA162311.0585333531592307No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAGAGGCCTGAGGGATCCATCTC147850.9642299073661034No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC126750.8266225279584282No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA114870.7491450081781826No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG96770.6311026590180442No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC87330.5695380305057952No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT79640.5193863363046092No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA79040.5154733302551019No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGANCCAATTTC76970.5019734593843015No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGCTATGAGAAACTCTCTAT72370.4719737463380785No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA72320.47164766250061957No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCANCTGGACAG59760.3897354025309323No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAANAAGTTAAC58940.3843876275966056No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT58610.38223547426937654No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT55090.35927917211226673No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCANGAATGCCG43250.28206251940198834Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA42690.27841038042244814No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATAACTCCATG40420.263606174201812No Hit
TGACTCTGAGATGCTCTCCTAAGTCTGGGCATGACACTGTGTCCTGGTAC37750.24619329728150424No Hit
AGGAACACCAGTGACTCTGAGATGTCATCAGACTGAGAACCACCGCTATA37560.24495417869916025No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT36370.23719338336763737No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGAACCAATTTC35080.22878042036119656No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGCAGTTAAC29350.19141121258840132No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGCAATGCCG29100.1897807934011066Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA28930.18867210835374618No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATNCAATTTCA28530.18606343765407463No Hit
TGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGAGA25840.16852012719878331No Hit
GATCACAGAGACAGGAAGGCAGACATAGAGCACCAGGCAGACCAACCAAA24870.16219410075207977No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGNCACTGTCT24130.15736805995768738No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAAGAATGCCG23710.15462895572303223Illumina Paired End PCR Primer 2 (96% over 31bp)
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGACAGAAGTTAAC22570.14719424422896824No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC22310.14549860827418173No Hit
CAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT21870.142629070504543No Hit
AGGGGACAGGATGTAACTCTCAGGTGTGATCCAATTTCGGGTCATGTATC21300.13891171475751102No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTACCCAGGATATGAACC21090.13754216264018343No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATACAATTTCA19660.1282161648888576No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAAAAGTTAAC19170.1250205432817599No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCGACTGGACAG18940.12352055762944877No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTAGACAAAT17710.11549889522795868No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA16020.10447726152184632No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCATGAATGCCG15900.10369466031194484Illumina Paired End PCR Primer 2 (96% over 31bp)
CTGATGTTACCCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCC15620.10186859082217474Illumina Paired End PCR Primer 2 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)