FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2308418
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT578452.5058286670784926Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGA72650.0283.452458
GGTAGAT76200.0265.02399
TCTGGTA80450.0262.20026
CTGGTAG86400.0240.9027
TTGGTAA14750.0234.85257295
TCGGTAA16350.0233.50784295
CCAGTAA25950.0229.48984295
GTACCAA25550.0222.69775295
CTGGTAA22600.0211.97975295
CAGGCTA7600.0195.89674295
TCGATAA14950.0190.29858295
TCGGAAA10650.0188.23865295
TGGCGTG19250.0164.636896
TGGGATG14400.0153.549446
TAACGTG4050.0152.866996
TCGCATG5700.0152.579656
TAGCGTG5950.0151.123616
TGGGGTG56100.0149.509526
GTCCAGA14550.0148.92712295
TTGTCGA81750.0146.23541295
Produced by FastQC (version 0.11.5)