FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107589
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT12598211.374435824118875Illumina Single End PCR Primer 1 (100% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGCAGAGCGTCGTGTAGGGAAAGAGT48890.4414092230962929Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTCGGGAAAGAGT15530.14021446583525116Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGA144950.0290.502328
GGTAGAT145800.0288.60649
CTGGTAG146200.0288.22037
GGGGTCT148700.0286.746982
GGGTCTG149050.0286.07363
TGGGGTC155750.0282.9611
TCTGGTA149900.0282.385256
GGCCTGT79100.0258.09534295
CTGTTGG97700.0234.47548295
TGGACCC109400.0220.3207295
GGTCTGG206500.0205.985614
GGCCGGT1700.0199.57193295
GGCCCGT3250.0186.08914295
TTTAGGC900.0180.26529
TTATGGG603.6379788E-12172.071328
TGGCGTC4550.0171.801216
TCGGTAA2600.0170.20348295
GGTGATT451.7871571E-8163.877449
TACGGTG3250.0163.373216
TGGCGTG10350.0162.452426
Produced by FastQC (version 0.11.5)