FastQCFastQC Report
Wed 8 May 2024
EGAF00005400945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383198
Total Bases115.3 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT9331224.350857781094888No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG5464014.258947071748809No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC4697812.259458556673051No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG4316211.263628724575806Illumina Paired End PCR Primer 2 (100% over 31bp)
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA200525.232803929039296No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC179744.69052552466349No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT60921.589778652289417No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT47371.2361755541521615No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA38791.0122704189479068No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT31550.8233341510133142No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC29870.7794925860782154No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT27720.7233858214291307No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGNCACTGTCT26050.6798052181900741No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA23260.6069969049942849No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT23080.6022995944655244No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT21190.5529778339135382No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCANCTGGACAG16480.4300648750776361No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG15160.3956179312000585No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA14850.38752811862274855No Hit
GATCACAGAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGA14740.38465753996628377No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGANCCAATTTC13460.3512544428728751No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA12970.33846731976680466No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCANGAATGCCG11960.3121101884665369Illumina Paired End PCR Primer 2 (96% over 31bp)
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATAACTCCATG11710.3055861460654805No Hit
TGACTCTGAGATGCTCTCCTAAGTCTGGGCATGACACTGTGTCCTGGTAC10950.28575305716626914No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA9500.2479136112401422No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA8310.21685916941111386No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT8250.21529339923486032No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA8240.21503243753881804No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGCAATGCCG8150.21268378227443777Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA7960.2077255100496349No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC6830.1782368383968601No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAAGAATGCCG6630.17301760447601502Illumina Paired End PCR Primer 2 (96% over 31bp)
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGAACCAATTTC6480.1691031790353812No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATNCAATTTCA5650.14744335826387403No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACTACACTGTCT5590.1458775880876205No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGTCACTGTCT5090.13282950328550774No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAANAAGTTAAC5060.13204661819738098No Hit
GATCACAGAGACAGGAAGGCAGACATAGAGCACCAGGCAGACCAACCAAA5030.1312637331092542No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGCTATGAGAAACTCTCTAT4990.1302198863250852No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAGAGGCCTGAGGGATCCATCTC4480.1169108398269302No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCGACTGGACAG4450.11612795473880343No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT4390.11456218456254992No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTAGACAAAT4370.1140402611704654No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACCACACTGTCT4290.11195256760212736No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCATGAATGCCG4290.11195256760212736Illumina Paired End PCR Primer 2 (96% over 31bp)
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATACAATTTCA4260.1111696825140006No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCTACTGGACAG3860.10073121467231039No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)