FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603610
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA14489024.00390980931396No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG10446917.307367339838635No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC7915213.113102831298354No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT6102510.1100048044267No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG408146.761650734745945Illumina Paired End PCR Primer 2 (100% over 31bp)
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC292604.847500869766903No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT154262.555623664286543No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA124692.0657378108381237No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT90441.4983184506552245No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA70311.1648249697652457No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG55400.9178111694637265No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA53000.8780503967793775No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA49740.8240420138831365No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC46780.7750037275724392No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT46060.7630754957671344No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA35230.5836550090290088No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC33300.5516807209953446No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA31980.5298122960189526No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT31100.5152333460346913No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC28550.4729875250575703No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT27890.4620533125693742No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA17440.2889282815062706No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA17080.28296416560361826No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT15070.2496645184804758No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT13880.229949802024486No Hit
GAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGTG13240.21934692930865957No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTACA12830.21255446397508326No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTATA11100.1838935736651149No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT10650.17643842878679944No Hit
GAGTCACACAAAGTCCCACACACCTGATCAAAACGAGAGGACAGCAAGCG9460.15672371233080964No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTACCCAGGATATGAACC9280.15374165437948345No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGTGCACCAGACAAATCA9220.1527476350623747No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATAACTCCATG8870.14694918904590715No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACATGACTAC8410.13932837428140687No Hit
TGACTCTGAGATGCTCTCCTAAGTCTGGGCATGACACTGTGTCCTGGTAC7810.12938818111031958No Hit
CAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT7660.12690313281754775No Hit
TGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGAGA6490.10751975613392753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCTT4750.0294.985784
ATTCTGG108200.0292.941041
TTCTGGA108300.0292.806732
TCAATGG19250.0291.920969
TTGCGCT4800.0291.912969
AGGTCAC160350.0289.55881
GCCACTC62700.0289.10498
CTTGCGC4850.0288.903568
ATCTACC45800.0288.5455
AAAGTCG91200.0288.516751
GGAGTCA109850.0288.406656
GTCGCAA91350.0287.558654
AGTCGCA91800.0286.309723
TCGCAAA91650.0286.134585
CGCAAAG91750.0285.983466
TCTTGCG4900.0285.955577
GAGTCAC111000.0285.551547
TACCAGA46450.0285.459848
AACAGCC63550.0285.00594
CATCTAC46450.0284.824774
Produced by FastQC (version 0.11.5)