FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603610
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT382806.341843243153692Illumina Single End PCR Primer 1 (100% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGCAGAGCGTCGTGTAGGGAAAGAGT14850.24601978098441044Illumina Single End PCR Primer 1 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGA45700.0289.156988
GGTAGAT45700.0288.511549
TCTGGTA47900.0276.18426
CTGGTAG48900.0269.631477
TTGGTAA12050.0253.37349295
TCGGTAA4550.0239.88231295
CTGGTAA7300.0230.33496295
GGGGTCT64700.0206.977682
GCGGTAT500.0206.476187
GGGTCTG64750.0206.134493
CGTCCGT650.0204.207214
TGGGGTC68300.0204.140961
GCGGTTA150.0028503465196.643982
GTACCAA3600.0192.56317295
GGTACGC1000.0191.727879
TTTAATG1000.0191.727876
ATGGCGT1850.0191.329285
TGAGGTG4600.0190.044541
CCAATTT408.8384695E-9184.353712
GGTGATC800.0184.353719
Produced by FastQC (version 0.11.5)