FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355709
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT6078717.088968791905742Illumina Single End PCR Primer 1 (100% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGCAGAGCGTCGTGTAGGGAAAGAGT21080.5926192477558904Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGGTAACATCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG15530.4365928329055492Illumina Single End PCR Primer 1 (100% over 30bp)
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTCGGGAAAGAGT7040.19791458748583812Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGACAGAGT4120.11582501426728029Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGG108.465328E-4294.967969
TGGTAGA68900.0291.54318
CTGGTAG69500.0288.389537
GGTAGAT69950.0286.534279
TCTGGTA70350.0286.162936
GGGGTCT74750.0278.197172
TGGGGTC76450.0276.765721
GGGTCTG75450.0275.029753
GGTCTGG80050.0258.304234
TGGGTAA2350.0244.760628
GGGTAAC2400.0239.661489
GGGTTTT202.2817876E-5221.225972
ATTGGCG353.974492E-9210.691394
GCCGTAA2350.0200.85747295
TGGGCTA1250.0200.578226
CGTGGTA457.8216544E-11196.645319
TCTTAGG150.0028497989196.645318
TTAGTGG150.0028497989196.645312
CGGCTTA304.6682544E-7196.645312
AGGTGCG150.0028497989196.645313
Produced by FastQC (version 0.11.5)