FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002870
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT749987.478337172315455Illumina Single End PCR Primer 1 (100% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGCAGAGCGTCGTGTAGGGAAAGAGT27100.2702244558118201Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGA85600.0290.157568
GGTAGAT85800.0289.309339
CTGGTAG87700.0282.705147
TCTGGTA90250.0277.005286
GGGTCTG104900.0237.757223
GGGGTCT105700.0236.515882
TGGGGTC111300.0233.45851
GGCCTGT60100.0207.13582295
TCGGTAA6400.0193.59163295
CTGTTGG41650.0183.08923295
TTGGGTA2900.0172.920526
CTGTCGG50200.0172.76701295
TTGGTAA3350.0171.71454295
GGCGTGC1650.0169.838132
CGCGTTA351.0068179E-6168.561172
TCGCATG1800.0163.878926
GGCGTTA451.7867933E-8163.878927
TTAGGGT2650.0161.405265
TTACGTG1950.0158.836496
GGGATTT1300.0158.836492
Produced by FastQC (version 0.11.5)