FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15246
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA301319.76256067165158No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG261517.152039879312607Illumina Paired End PCR Primer 2 (100% over 31bp)
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC14899.766496130132493No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT13528.867899776990686No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG9736.382001836547291No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC7675.030827758100485No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT4603.0171848353666535No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA3011.9742883379247016No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA2651.73816082906992No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT2631.7250426341335432No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA2621.7184835366653546No Hit
AAGTGATCTTGCGCTGTGTCCCACGCCAAGTCAACCTCCCATGGCGGGGC2101.3774104683195594No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT1861.2199921290830382No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG1831.200314836678473No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGAGCATGACACTGA1430.937950937950938No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC1390.9117145480781845No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA1310.8592417683326773No Hit
GATCACAGAGACAGGAAGGCAGACATAGAGCACCAGGCAGACCAACCAAA1310.8592417683326773No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC1030.6755870392234029No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA480.3148366784730421No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT470.3082775810048537No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA430.28204119113210024No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC420.27548209366391185No Hit
AAGTGATCTTGCGCTGTGTCCCTCTCACTTCCCTGCATCACTGTGTGTGT380.24924570379115832No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT310.2033320215138397No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA300.19677292404565133No Hit
GGAAACAGCCACTCTGAAATGCAGCCAACCCATGGTAAGCGAGCCAGAAC300.19677292404565133No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT290.19021382657746294No Hit
TGACATCTACCAGACCCCAAGATCGGAAGCGCGGTTCAGCAGGAATGCCG280.18365472910927455Illumina Paired End PCR Primer 2 (96% over 31bp)
AAGTGATCTTGCGCTGTGTCCCTGCCACGTGAAGGTCCTGGCTCCAGCTC280.18365472910927455No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT240.15741833923652104No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA240.15741833923652104No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATAACTCCATG240.15741833923652104No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAGAGGCCTGAGGGATCCATCTC200.13118194936376756No Hit
AAAGTCGCAAAGAGAGGACAGGTGTGCCCAGGCATGCCCCGGGGGTGTGA190.12462285189557916No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA190.12462285189557916No Hit
CAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT180.11806375442739078No Hit
GATCACAGAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGA180.11806375442739078No Hit
CTGATGTTACCCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCC180.11806375442739078Illumina Paired End PCR Primer 2 (100% over 30bp)
AAGTGATCTTGCGCTGTGTCCCCTGTTCCCTTCCCTGGTCCTGACATCCA170.1115046569592024No Hit
AGGAACACCAGTGACTCTGAGATGTCATCAGACTGAGAACCACCGCTATA170.1115046569592024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATCA255.0567905E-10295.05
TGATCTT500.0295.04
ATAGGAC157.1009217E-6295.08
TCAATGG255.0567905E-10295.09
GAGTCAC1000.0295.07
AAAAGCC303.6379788E-12295.08
AGGCTCC205.9875674E-8295.0295
TCTTGCG500.0295.07
TATTCAT255.0567905E-10295.03
CTTGCGC500.0295.08
AAGTGAT500.0295.01
TTGCGCT500.0295.09
ATTCTGG1000.0295.01
AAAGCCA303.6379788E-12295.09
ATTCATC255.0567905E-10295.04
GATAGGA157.1009217E-6295.07
GTACGTC157.1009217E-6295.0295
AGATAGG157.1009217E-6295.06
CACGGCC108.3831814E-4295.0295
AAACAGC1300.0294.999973
Produced by FastQC (version 0.11.5)