FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792075
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG17098921.587475933465896No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC14637318.479689423350063No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT13608717.181075024461066No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA746929.429915096423949No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG429975.4284000883754695Illumina Paired End PCR Primer 2 (100% over 31bp)
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT227922.877505286746836No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT183602.317962314174794No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT147241.858914875485276No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA135131.7060253132594765No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT105341.3299245652242526No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA103031.3007606602910078No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTACA81751.0320992330271754No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC72090.9101410851245149No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA63630.8033330177066567No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA60200.7600290376542626No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT58410.7374301675977654No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT46210.5834043493356059No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC39980.5047501814853391No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGTGATCGCTTCTTTGC37630.4750812738692674No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTACACTTCTATTGT36120.4560174225925575No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGTGCACCAGACAAATCA32240.4070321623583626No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA27050.3415080642615914No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC24970.31524792475460023No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG20470.2584351229365906No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT18110.2286399646498122No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACATGACTAC17080.21563614556702332No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA15180.19164851813275258No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTACTCACTTATACTTCTATTGT14920.18836600069437867No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA14890.18798724868225863No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA13750.17359467222169617No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGTCTCCACT13680.17271091752674936No Hit
AGGAACACCAGTGACTCTGAGATGTCATCAGACTGAGAACCACCGCTATA13200.16665088533282835No Hit
CAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT12220.15427831960357288No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGA11800.14897579143389197No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT10840.13685572704604992No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTAGACAAAT10640.13433071363191618No Hit
TACAGAGAAAAGCCAGGCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG9910.12511441467032794No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGATCCGTCTC9250.11678187040368652No Hit
TGACTCTGAGATGCTCTCCTAAGTCTGGGCATGACACTGTGTCCTGGTAC8940.1128680996117792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTGC400.0295.0295
ATTCTGG178750.0293.927251
GAGTCAC178700.0293.266637
TTCTGGA179100.0293.10582
GCCACTC137400.0292.423588
GGAGTCA179250.0292.366826
AGTCACA179600.0291.879188
GTCACAC179800.0291.80069
AAAGTCG204950.0291.47351
AAGTGAT32800.0291.402441
ATCTTGC33150.0290.995486
GATCTTG32850.0290.95895
TCTTGCG33200.0290.557257
TCGCAAA205250.0290.185125
CTTGCGC33200.0290.112988
ACAGCCA138750.0290.109925
TTGCGCT33150.0290.10569
GTCGCAA205850.0290.055854
AGTCGCA206050.0289.774323
CGCAAAG205750.0289.479956
Produced by FastQC (version 0.11.5)