FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825782
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC42941252.000649081718905No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG15338018.573909336846768Illumina Paired End PCR Primer 2 (100% over 31bp)
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA546316.615668542060737No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA199432.4150441641982994No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT155071.8778563834038524No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG133871.6211300318970383No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT128031.5504091878970478No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT122351.4816259012669202No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT87731.0623869253628682No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGCTCTCAGGTGTGATCCAATTTCG66470.8049339898423555No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAA62070.7516511621711299No Hit
CAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT60930.7378460659108579No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT44610.5402152141848575No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGAGCTCAGGTGTGATCCAATTTC32660.3955038981232335No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA31990.3873903766369332No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC27810.3367716903492689No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA19520.23638199912325528No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGTGATCGCTTCTTTGC17690.2142211867054501No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTAGACAAAT16840.20392791317805423No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT14870.18007173806161916No Hit
TGACATCTACCAGACCCCAAGATCGGAAGCGCGGTTCAGCAGGAATGCCG14260.17268480058901745Illumina Paired End PCR Primer 2 (96% over 31bp)
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA13480.16323920841093653No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCG12690.15367251889723924No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGTCTCCACT10790.13066402513011907No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA9780.1184331942328605No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGATCCGTCTC9140.11068296475340948No Hit
TGACATCTACCAGACCCCAAGCTCGGAAGAGCGGTTCAGCAGGAATGCCG8370.101358469910945Illumina Paired End PCR Primer 2 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACATC168650.0289.570561
AAAGTCG505000.0288.947021
GTCGCAA505300.0288.571144
ATCTACC168700.0288.435585
AGTCGCA506100.0288.056733
TCGCAAA505350.0288.01725
AAGTCGC505900.0287.995642
CGCAAAG505300.0287.928966
CTACCAG169200.0287.408877
GCAAAGA507950.0286.5727
TACCAGA169900.0286.398358
TCTACCA170050.0286.145756
AGGTCAC73050.0285.906921
CTTGCGC5650.0284.55078
CATCTAC171550.0284.07364
AAAGAGA514450.0282.37789
ACATCTA172800.0282.274753
GACATCT172900.0282.11152
TTGCGCT5700.0282.054669
TCTTGCG5850.0274.822487
Produced by FastQC (version 0.11.5)