FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825782
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT14687217.7858078766551Illumina Single End PCR Primer 1 (100% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGCAGAGCGTCGTGTAGGGAAAGAGT50190.6077875274588209Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTCGGGAAAGAGT15510.18782196754107014Illumina Single End PCR Primer 1 (96% over 31bp)
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGACAGAGT8640.10462809797258842Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTCT168350.0292.52662
TGGTAGA166950.0291.534248
GGTAGAT167650.0290.404979
CTGGTAG167550.0290.402227
GGGTCTG169650.0290.111153
TGGGGTC173300.0288.6111
GGTCTGG172500.0284.9764
TCTGGTA173150.0282.032236
CTGTTGG224250.0279.60635295
CTGTCGG20200.0246.07465295
CCGTTGG8800.0222.92426295
GTCTGGT251600.0195.207125
CTGGTGG12100.0191.38269295
TGGGGTA5600.0182.490981
ATGCGGT1700.0182.193315
TTGGGTA1950.0173.962376
ATTAGGT1900.0170.77775
CTTTTGG5300.0164.19672295
GGGTAAC1550.0161.763039
GTGAGGT3300.0160.8985
Produced by FastQC (version 0.11.5)