FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230898
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT3121113.517224055643617No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA186598.081057436617034No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA166047.191054058502022No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT138996.019541096068394No Hit
AAGTGATCTTGCGCTGTGTCCCGCGAGGAGCTCATGATGGTGCTGGCCAG127705.530580602690365No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT98024.245164531524743No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT93034.029051789101682No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC92844.020823047406214No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC91823.9766476972516No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT81953.5491862207554856No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA70083.0351064106228725No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG56822.460826858612894Illumina Paired End PCR Primer 2 (100% over 31bp)
AGGAACACCAGTGACTCTGAGATGTCATCAGACTGAGAACCACCGCTATA56792.4595275836083466No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT49572.1468353991805906No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG47692.0654141655622826No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC47242.045925040494071No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA45391.965803081880311No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA41031.7769751145527461No Hit
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT37161.6093686389661237No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTACTCACTTATACTTCTATTGT27611.195766095851848No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA26191.134267078969935No Hit
GATCACAGAGACAGGAAGGCAGACATAGAGCACCAGGCAGACCAACCAAA24341.0541451203561747No Hit
TGACTCTGAGATGCTCTCCTAAGTCTGGGCATGACACTGTGTCCTGGTAC19670.8518913113149529No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA18740.8116137861739816No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA17930.7765333610512002No Hit
GACAGAGCATGACACTGCTGTGTGTTTTGTGTGTGTGTTGTGTGTGTGCT16790.7271609108783965No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC16320.7068056024738196No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGCTATGAGAAACTCTCTAT15950.6907812107510676No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGTGCACCAGACAAATCA13720.5942017687463729No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT11170.48376339335983853No Hit
GAACCCCAGACACAAGATCACAAAGAGGGGACAGAATGTAACTTTCAGGT10960.4746684683280063No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG10860.47033755164618146No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA9340.40450761808244334No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGTCTCCACT6800.2945023343640915No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGATCCGTCTC6470.28021030931406943No Hit
GAGGACAGCAAGTGACACTGAGCTGCTCCCCTATCTCTGGGCATAGGAGT4830.2091832757321415No Hit
CATGAGGTGACAGAGATGGGACAAGAAGTGACTCTGAGATGTAAACCAAT4480.1940250673457544No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT4320.1870956006548346No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTAGACAAAT4080.1767014006184549No Hit
TGAAGACAGGACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCAT3840.1663072005820752No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC3280.1420540671638559No Hit
TGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGAGA3250.14075479215930845No Hit
GACAGAGCATGACACTGCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG2860.12386421710019144No Hit
GTACAAAGTCGCAAAGAGGGGACAGGATGTAGCTCTCAGAAAGGCCTGAG2780.12039948375473153No Hit
CAAAGAGGGGACAGAATGTAACTTTCAGGTGTGATCCAATTTCTGAACAC2600.11260383372744674No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCCATTTCAGGCCACAACTCC2390.10350890869561451No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGCG108.460654E-4295.06
CTTGCTT900.0295.0295
TATTCAT5100.0295.03
AGTGTCA255.293259E-10295.09
TCTTGCG48500.0293.47947
GTGATCT48850.0293.188323
GATCTTG48700.0293.182775
TGATCTT48900.0292.58694
CTTGCGC48650.0292.574528
ATCTTGC48650.0292.574526
TTGCGCT48650.0292.574529
CATCTAC5550.0292.342354
AACAGCC10550.0292.20384
TCAATGG5200.0292.163459
AACCTCT17350.0291.599461
TCTGTGA17300.0289.88445
CTGTGAA17300.0289.88446
ACAGCCA10650.0289.46015
GCCACTC10650.0289.46018
AAAGTCG37400.0288.295441
Produced by FastQC (version 0.11.5)