FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005400960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230898
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT50452.1849474659806494Illumina Single End PCR Primer 1 (100% over 31bp)
TTCGGTGGGATGGACTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA45251.9597397985257559No Hit
TACGCTCTGCTGTCTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA35871.5534998137705827No Hit
TTCTTTGCCCTTCTATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA27391.1862380791518332No Hit
TGGTATTGTATCAGCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA27111.1741115124427235No Hit
TTTTATCACATTCCTTTCTGATGGCTCAAACACAGCAAGGAAGGGAACAC18620.8064166861557918No Hit
TATACTGTTTTGTTATTCTGATGGCTCAAACACAGCAAGGAAGGGAACAC18610.8059835944876091No Hit
TTAGCTTCCATCATGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA17720.7674384360193679No Hit
TGTGGTGGGGTACGCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA14920.6461727689282714No Hit
TTAGTTCCGGTCCGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA11690.5062841601053278No Hit
TGTACTTGCTTAAGTTTCTGATGGCTCAAACACAGCACACACACAACACA10180.4408873182097723No Hit
TTCACTAATTTAACCTTCTGATGGCTCAAACACAGCAAGGAAGGGAACAC10000.4330916681824875No Hit
TCCGCTTCCATTAGCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA9370.4058068930869908No Hit
TTGTATGTAATTCATTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA8540.3698602846278443No Hit
TAAGTTGTTTTACGCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA7890.34170932619598265No Hit
TGGTCTTTAATTGCGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA7890.34170932619598265No Hit
TGACGTAAGGTGGGTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA7770.3365122261777928No Hit
TTTGTTCCGTTGCGTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA7340.31788928444594583No Hit
TGTCGTGCATTCCCGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA7230.3131252760959385No Hit
TAGGGTATGTTACCGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA6510.28194267598679934No Hit
TGATTTGATGTGGAGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA6440.2789110343095219No Hit
TTGCGTTAAATGTCATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA6320.2737139342913321No Hit
TAAAGTGTACTAATGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA6180.26765065093677726No Hit
TAATTTACTATGCCATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5900.25552408422766765No Hit
TACGATCCTGTCTCCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5510.23863350916855064No Hit
TCGCATCCGGTCGGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5510.23863350916855064No Hit
TCGTGTCCCGTCCAATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5500.23820041750036813No Hit
TGCCGTTATGTGTGATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5420.23473568415490823No Hit
TTGTTTTATTTAACGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5390.23343640915036074No Hit
TATGCTCAACTTGGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5210.225640759123076No Hit
TAGCTTCATATCGATTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5140.2226091174457986No Hit
TTGCGTTTTGTGCTGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA4800.207884000727594No Hit
TGTTATAGTTTAATGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA4790.20745090905941152No Hit
TGTGTTGAGTTCGGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA4750.20571854238668158No Hit
TGGCATTGCGTCGGATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA4560.19748980069121427No Hit
TTTAGTTCGTTGTGATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA4560.19748980069121427No Hit
TGAGGTCGCGTCCAGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA4560.19748980069121427No Hit
TGGGGTGGGGTGTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA4460.19315888400938944No Hit
TACACTGTGCTCGCTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA4400.19056033400029448No Hit
TTGAGTATCTTACGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA4200.18189850063664476No Hit
TCTGTTGGGATTGGCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA4020.17410285060935998No Hit
TTCACTTCCATTAGTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA3870.16760647558662267No Hit
TGGTGTAATTTCCGTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA3720.16111010056388536No Hit
TTCGGTGGGCTCCTATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA3540.15331445053660056No Hit
TAGATTTAGTTAAGATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA3530.15288135886841808No Hit
TGATTTCTTGTACTCTTCTGATGGCTCAAACACAGCGGTGGGGTGGGAAC3480.15071590052750566No Hit
TTGTATTTATTTCCCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA3450.1494166255229582No Hit
TAAACTGATCTCAGATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA3370.1459518921774983No Hit
TTGGGTTGAGTGGAATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA3370.1459518921774983No Hit
TTTATTGAATTACCATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA3240.14032170049112597No Hit
TTATATGCGGTGGGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA3180.13772315048203104No Hit
TCGGTTGAGTTGGCCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA3170.13729005881384854No Hit
TGGGCTTATGTGGCCTTCTGATGGCTCAAACACAGCAAGGAAGGGAACAC3030.1312267754592937No Hit
TCCCTTCATCTTTTATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2970.1286282254501988No Hit
TCTGGTGTTATGATTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2950.12776204211383382No Hit
TGGACTCGACTGTAATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2940.12732895044565132No Hit
TTTCATCCGATCCGTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2880.1247304004365564No Hit
TGATATGGGATCGCCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2870.1242973087683739No Hit
TGGCCTGGCTTGTGATTCTGATGGCTCAAACACAGCAAGGAAGGGAACAC2800.12126566709109649No Hit
TGTCGTGCACTCCGTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2740.11866711708200157No Hit
TGGTCTTGGTTTTCGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2700.11693475040927162No Hit
TCGGATCGGTTGCGCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2500.10827291704562188No Hit
TGACTTGGCGTATTCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2480.10740673370925691No Hit
TAATTTGGCGTGGCGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2480.10740673370925691No Hit
TGTCATCGGGTATCTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2460.10654055037289192No Hit
TCGGGTTGTTTGGGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2460.10654055037289192No Hit
TTTCCTGGCTTTCATTTCTGATGGCTCAAACACAGCAAGGAAGGGAACAC2430.10524127536834446No Hit
TGGCGTGCGGTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA2400.103942000363797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)