FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520219
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGT8018715.41408522180082No Hit
GGAAACAGCCACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCT6734612.945701714085796No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCA459028.823591602767296No Hit
ATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAG431938.302849376897038No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTC288035.536706656235162No Hit
TGAAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT217984.190158375607196No Hit
AACCTCTGTGAAGATCGAGTGCCGTTCCCTGGACTTTCAGGCCACAACTA185513.565998166156945No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGAT182463.5073690118969125No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGG178473.430670544520673No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGGCAACAGTGCACCAGA169173.2518996807113925No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTA153322.947220305294501No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCAGGACGGCGCATGACACTGA132642.5496954167379506No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCACAACTCC111772.1485182202111033No Hit
GTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAAGTTAAC90921.7477254771548136No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGT88951.7098568103048908No Hit
AGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGCTACA71481.374036703772834No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTACTCACTTATACTTCTATTGT51250.9851620183038297No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATGA48910.9401809622485914No Hit
TGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGTACTGGT48400.9303773987493729No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGTGCACCAGACAAATCA42100.8092745555237313No Hit
ACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTGGGCAGCCCGCT39180.7531443488223228No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGA38640.742764105117268No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACC33850.6506874989187246No Hit
TGACATCTACCAGACCCCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCG32070.616471140039099Illumina Paired End PCR Primer 2 (100% over 31bp)
AGTGACTCTGAGATGCTCTTCTCAGTCTGGGCACAACACTGTGTCCTGGT25590.49190821557843906No Hit
GACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCATGAATACATG21630.41578642840803587No Hit
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCATAA20990.4034839173501929No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGA20380.39175808649818633No Hit
TGACTCTGAGATGCTCTCCTAAGTCTGGGCATGACACTGTGTCCTGGTAC16720.32140310138614697No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGAGAGGACTGGGGAATC15100.29026237027098206No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAACTTTAAAGAACAGACTGA13720.2637350808025082No Hit
GATCACAGAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGA12090.23240212295206442No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACATGACTAC11270.21663953065920316No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAATGAAGCTCAACTAGACAAAT10930.2101038216597241No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTACTCACTTATACTTCTATTGG10900.20952714145388768No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT10310.19818576407243874No Hit
CCTGAAGATAGGACAGAGCATGACACTGCAGTGTACCCAGGATATGAACC10200.19607126998437197No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTGC9360.1799242242209531No Hit
GACGATCCAGTGTCAAGTCGATAGCCAAGTCACCATGATGTTCTGGTACC8650.1662761260161586No Hit
TGAAGACAGGACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCAT7330.1409021969593575No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGAAAGGCCTGAGGGATCCGTCTC7300.14032551675352112No Hit
TGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACACGACTAC7200.13840324940073315No Hit
AAGAAGCCACTGGAGCTCATGTTTGTCTACAGCTATGAGAAACTCTCTAT6820.1310986334601389No Hit
GAGGACAGCAAGTGACACTGAGCTGCTCCCCTATCTCTGGGCATAGGAGT6240.11994948281396874No Hit
AAAGTCGCAAAGAGAGGACAGGATGTAGCTCTCAGTGATCGCTTCTTTGC6120.11764276199062319No Hit
AAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTTATACTTCTATTTT5940.11418268075560487No Hit
CAAAGAGGGGACAGGATGTAGCTCTCAGGCCTGAGGGATCCGTCTCCACT5510.10591693113861662No Hit
AGGTCACAGAGAAGGGAAAGGATGTAGAGCTCAGAGAGGACTGGGGGATC5450.10476357072694384No Hit
CATGAGGTGACAGAGATGGGACAAGAAGTGACTCTGAGATGTAAACCAAT5400.10380243705054987No Hit
CCAGGCACCAACATCTCAGATCCTGGCAGCCGGACGGCGCATGACACTGA5230.10053458255081033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTAGG255.311449E-10295.0295
TATCTTG850.0295.05
CGCAACG157.238088E-6295.06
CACGCTT108.4636983E-4294.99997295
GGTGACA400.0294.999976
ATCTTGC118800.0294.751686
GATCTTG118000.0294.755
TGATCTT118000.0294.754
GTGATCT118200.0294.376073
TCTTGCG118900.0294.255687
TTGCGCT118800.0294.255049
AAGTGAT118150.0294.250951
CTTGCGC118900.0294.131628
GCCACTC72650.0292.76678
ACAGCCA72800.0292.56875
ATTCTGG43100.0292.26221
CTGTGAA20350.0292.100746
AAACAGC73100.0291.973333
AACAGCC73200.0291.171454
TTCTGGA43450.0290.24742
Produced by FastQC (version 0.11.5)