FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005400962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005400962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520219
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGTCTGGTAGATGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT27520.5290079754872468Illumina Single End PCR Primer 1 (100% over 31bp)
TCACATGGCTTTGCCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA11620.22336746639396102No Hit
TACTTTGACTTGCGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA8270.15897151007556432No Hit
TGATATGTGTTCTGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA7960.15301248128192166No Hit
TGCGCTGGGATGGCGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA7710.14820681289995175No Hit
TTCCATGTCATAGTCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA7510.14436227819437583No Hit
TGTCATATTGTACCTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA7070.1359043018421088No Hit
TTCGTTATGGTGACTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA6920.13302090081292686No Hit
TCAGCTCGCTTCGACTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA6570.12629296507816898No Hit
TTGCCTGCGGTAGGGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA6370.12244843037259308No Hit
TTGCCTACCATGGCCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA6220.11956502934341114No Hit
TGTGATCGCGTAGAGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5920.11379822728504725No Hit
TTAACTCGGGTAGGCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5790.11129927972642292No Hit
TTCGCTCACGTTCGCTTCTGATGGCTCAAACACACGACCTCGGGTGGGAA5740.11033814605002892No Hit
TTCGGTTCCTTTTCATTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5720.10995369257947134No Hit
TGAGGTAGCTTGGCGTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5650.10860810543251975No Hit
TGCGATAGAGTATGCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5620.10803142522668337No Hit
TGTTCTTGACTACATTTCTGATGGCTCAAACACACGACCTCGGGTGGGAA5240.10072680928608913No Hit
TCCCGTGGGTTTGCTTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGA5210.10015012908025274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG157.238088E-6295.09
GAGTATG500.0295.08
GGTCAAC255.311449E-10295.04
TTAGTGG255.311449E-10295.02
TGGCTAT157.238088E-6295.07
GGTACGC206.1816536E-8294.999979
ACCATGT206.1816536E-8294.999972
GGGTATA400.0294.999973
GGACTAT108.4636983E-4294.999977
TATGACT108.4636983E-4294.999974
CGGCTTA108.4636983E-4294.999972
CGGTATT108.4636983E-4294.999973
ATTGCGC108.4636983E-4294.999979
GGCTATC108.4636983E-4294.999978
CTTGCGG950.0279.47379
GCGATTG750.0275.33332
CGTTCGC1350.0273.148139
TCTCAAG550.0268.181824
TCTCGGG28200.0263.09396295
CGTGCGT800.0258.1259
Produced by FastQC (version 0.11.5)